TotalLab is a specialist software developer for biotech, pharmaceuticals, and life science industries, boasting over 20 years of experience. TotalLab offers advanced software services for equipment manufacturers, bespoke solutions for life science companies, and off-the-shelf products to tackle scientific challenges, automate workflows, and enable FDA 21 CFR Part 11/GMP regulatory compliance. Totallab provides the following software:

Phoretix 1D Phoretix Array Phoretix Colony Phoretix Toolbox CLIQS 1D Pro SameSpots SpotMap
CLIQS1DPro

Advanced Band Matching Alorithms for Reliable Analysis

Formally Phoretix 1D Pro, our CLIQS 1D Pro software is an innovative solution for scientists who work with electrophoresis gels and immunoblots. Using advanced algorithms and accurate band-matching capabilities, CLIQS 1D Pro helps users quickly and easily identify and match DNA/RNA or protein bands of interest across even complex gel and blot images. The software also includes advanced tools and features, such as automatic lane and band detection, background subtraction, and 1D gel lane normalization.

One of the key features of CLIQS 1D Pro is its powerful databasing capabilities. The software allows users to easily create and manage a comprehensive database of lanes and bands, including their molecular weights and other important characteristics. This makes it easy to track and analyse changes in protein expression over time, across different experimental conditions or identify unknown samples by comparing their profiles against those stored in your database. In addition, CLIQS 1D Pro is designed to be user-friendly and intuitive, with a streamlined workflow that simplifies the analysis process.


Compare lanes from multiple gels and experiments

The CLIQS 1D Pro software is incredibly powerful as it allows a completely flexible approach to matching. Each individual lane can be compared to any other lane within the database. The results of matching across many gels can be presented as a dendrogram or tables that show band and lane similarities.

CLIQS 1D Pro can be used to analyse a variety of research techniques including (but not limited to):

  • DNA Fingerprinting
  • Pulsed-Field Gel Electrophoresis (PFGE)
  • Denaturing Gradient Gel Electrophoresis (DGGE)
  • Restriction Fragment Length Polymorphism (RFLP)
  • Enterobacterial repetitive intergenic consensus (ERIC)-PCR results
  • Single-Strand Conformation Polymorphism (SSCP) Fingerprinting
  • Automated Approach for Ribosomal Intergenic Spacer Analysis (ARISA)-PCR
  • Amplified fragment length polymorphism (AFLP)
  • Inter simple sequence repeat (ISSR)-PCR

Identify lane relationships across multiple experiments

Our software allows you to easily visualise your matching results using dendrograms, which allow the study of relationships between lanes stored in the database.
Once built, the dendrogram is scalable and fully interactive, to allow a flexible presentation of lane relationships. The dendrogram also allows you to create groups of related lanes that can then be reported on in a cluster table.

Identify unknown samples

Lanes containing unknown samples can be matched against a defined reference library of identified samples. Maintenance of the library is very straightforward, and it can be easily shared with co-workers to facilitate collaboration on large projects. This is especially useful in projects based around food security and verification to ensure that the meat content of foods entering the supply chain is from the species claimed.



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